Autosampler Control
Injecting Two Samples Simultaneously

Some autosamplers (for example, an AS or AS-AP in the Simultaneous mode, or an AS-DV) can deliver sample to two independent ion chromatography (IC) systems. The two IC systems and the autosampler are configured into a single timebase and each system is assigned a unique device name and channel. This lets you monitor and control both systems from one Control Panel and run all samples in one sequence.

For plumbing connections, refer to the autosampler operator's manual or the simultaneous injection kit instructions.

To set up simultaneous injections in Chromeleon, follow these basic steps:

1. In the Server Configuration program, create a timebase that includes both systems and the autosampler.

2. Assign a unique name to each device in the timebase. For example, if the timebase includes two ICS-2100 systems, name the second system Pump_ECD_2. In addition to renaming the device, also rename any signals and other devices associated with system #2 (for example, the detector signals, TTLs, relays, and eluent generator).

    Tip: 

   Although you can assign any unique device and signal names to system #2, adding _2 to the default names will allow the timebase to be connected to the template control panels (described below) without errors.

3. In the Browser, open a simultaneous injection control panel and then connect the timebase to the panel. For example, to open a simultaneous injection control panel for a timebase with two ICS-2100 systems and an AS-DV Autosampler, go to the Dionex Templates\Panels\Dionex_IC\ICS-2100 System Panels folder and double-click the ICS-2100_System_Simultaneous_AS-DV.pan file.

4. Create sequences, PGM Files, and QNT Files for simultaneous injections. Follow these guidelines:

• Create one sequence that includes calibration standards for both systems and the unknown samples.

• Create a PGM File for the unknown samples that includes commands for controlling both systems.

• Calibrate each system separately by creating separate calibration PGM Files.

• Create a single QNT File that includes the components from both systems. Use the Duplicate Column command on the Peak Table, Amount Table, and Peak Tracking pages of the method to create two extra Ret. Time columns; associate each column with a different channel.

Also, refer to:

Autosampler Control  Autosampler Commands (AS/AS50)

Autosampler Control   Autosampler Commands (AS-AP)

Autosampler Control   Autosampler Commands (AS-DV)

How To: Integrating Chromatograms and Identifying Peaks  Defining the QNT Method for Several Detectors