Using a Mix Method (ESA 542 Autosampler): Program ExampleAutosampler
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Using a Mix Method (ESA 542 Autosampler): Program Example
The ESA 542 Autosampler allows you to perform a mix method prior to injection. A mix method can consist of up to 15 dispense, mix and/or wait steps. Use the PGM wizard to configure the steps in a Program.
The example below is a typical PGM File using a mix method. It was created using an ESA 542 autosampler, Corona CAD and ESA pump.
|
;Configuration of the mix method steps |
|
|
mmDispense_Vol_From_To |
10, SampleVial, DestinationVial ;Dispense 10µL from SampleVial to DestinationVial |
|
mmDispense_Vol_From_To |
10, ReagentAVial, DestinationVial ;Dispense 10µL from ReagentAVial to DestinationVial |
|
mmMix_Vol_Reps |
10, 2 ;Mix 10µL for 2 repetitions |
|
mmWait_Secs |
10 ;Wait 10 second(s) |
|
;Detector and pump settings |
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|
InjectionMode = |
PartialLoopfill |
|
FlushVolume = |
30 [µL] |
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VolumesPerWash = |
1 |
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SyringeSpeed = |
Normal |
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AnalysisTime = |
0 [sec] |
|
WashBetweenSamples = |
yes |
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UseAirSegment = |
no |
|
UseHeadPressure = |
no |
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UseOven = |
no |
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UseTrayCooling = |
no |
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DestVialOffset = |
40 |
|
Pressure.LowerLimit = |
0.0 [bar] |
|
Pressure.UpperLimit = |
340.0 [bar] |
|
%A.Equate = |
"%A" |
|
Data_Collection_Rate = |
10 [Hz] |
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CAD_1.FilterConstant = |
None |
|
Flow = |
0.5000 [ml/min] |
0.000 |
|
Autozero |
|
Wait |
CAD.Ready and Sampler.Ready |
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Inject |
|
|
CAD_1.AcqOn |
|
30.000 |
|
CAD_1.AcqOff |
|
End |
|
Application Tips
The mix method is limited to 15 steps.
A mix method can only be performed with an “84+3 vial” tray.
It is possible to perform a mix method without a destination vial (using the sample vial).
In order to add more volume than the loop volume, more than one aspiration step is required.
In addition to the program example above, a
list of autosampler commands is also available (see 
ESA 542 Autosampler).