Dionex Autosamplers
ESA 542 Autosampler

Chromeleon provides the commands and parameters from the table below (please note that the display Filter level determines which commands and properties are displayed).

General and Configuration Commands and Properties

Command/Property

Description

LoopVolume

Reports the loop volume defined in the Chromeleon Server Configuration.

NeedleToValveVolume

Reports the needle-to-valve volume defined in the Server Configuration.

SyringeVolume

Reports the syringe volume that is defined in the Server Configuration.

TrayType

Reports the tray type defined in the Server Configuration.

StartWash

Starts a manual wash.

StopWash

Stops a manual wash

VialType

Reports the vial type defined in the Server Configuration.

 

Injection-related Commands and Properties

AnalysisTime

Sets the analysis time in seconds. The analysis time can be used to delay the next injection for a specified amount of time, for example, to allow a detector to equilibrate between samples.

FlushVolume

Sets the flush volume used for FullLoop and PartialLoop injections.

InjectionMode

Determines the mode of sample injection.

Position

Specifies the position of the sample on the sample tray.

RunStatus

Reports the status of the run (read-only).

StopInject

Stops a manual sample injection.

SyringeSpeed

Sets the syringe speed (Low, Normal, or High).

UseAirSegment

 introduce a 5µL air segment between the sample and the wash solvent when loading the sample loop.

Use of an air segment is not recommended in MicroliterPickUp injection mode. In this mode, the air segment would travel to the chromatography column.

UseHeadPressure

Determines whether or not to use headspace pressure in the injection.

Volume

Reports the injection volume.

VolumesPerWash

Specifies the number of syringe volumes to use per wash.

WashBetweenSamples

Specifies whether or not to perform a wash between sample injections.

 

Temperature Commands and Properties

ActualOvenTemp

Reports the current temperature of the column oven.

ActualTrayTemp

Reports the current temperature of the tray.

NominalOvenTemp

Sets the nominal temperature for the column oven.

NominalTrayTemp

Sets the nominal temperature for the tray.

OvenAvailable

Specifies whether an oven is available or not.

OvenOnOff

Enables or disables the column oven.

TrayCoolingAvailable

Specifies whether tray cooling is available or not.

TrayCoolingOnOff

Enables or disables tray cooling.

UseOven

Specifies whether or not to use the column oven.

UseTrayCooling

Specifies whether or not to use tray cooling.

 

Mix Method Commands and Properties

CycleVolume

Indicates the volume to mix in a mix method.

DestVialOffset

Sets the number of vial positions between a sample and its destination vial.

MixFrom

Indicates where to dispense from (either from SampleVial, Wash, ReagentAVial or ReagentBVial)

MixTo

Indicates where to dispense to (either to DestinationVial or SampleVial).

MixVolume

Reports the volume dispensed in a mix step.

mmDispense_Vol_From_To

Performs a dispense step in a mix method.

Volume: Sets the volume to be dispensed.

From: Sets where to dispense from (from SampleVial, Wash, ReagentAVial or ReagentBVial).

To: Sets where to dispense to (to DestinationVial or SampleVial).

mmMix_Vol_Reps

Performs a mix step.

Retention Time: Sets the retention time.

CycleVolume: Sets the volume to mix.

Cycles: Sets the number of mix repetitions

mmWait_Secs

Performs a wait step in the mix method.

NumCycles

Indicates the number of mix repetitions

WaitSecs

Indicates the wait period.

 

System Wellness and Predictive Performance Commands and Properties

GetCountlog

Reports the syringe, syringe valve and injector valve countlog values.

InjectorValveCountlog

Reports the total number of injector valve switches.

SyringeCountlog

Reports the total number of syringe draws.

SyringeValveCountlog

Reports the total number of syringe valve switches.

 

Important:

It is critical that the destination vials be positioned properly, since the autosampler cannot recover from a missing destination vial. If a missing destination vial is detected, the autosampler will sound an audible alarm and Chromeleon will abort a running sequence.

It will then be necessary to press the <PANIC STOP> button on the autosampler's LCD panel, followed by the <SERIAL> button, to recover from this condition.

 

For additional autosampler commands and a program example, refer to:

 General Device Commands

Using a Mix Method (ESA Autosampler 542): Program Example

For an overview of the control commands provided for the Dionex/ESA devices, refer to  Commands for Controlling Dionex Devices.

For installation and configuration details, refer to  ESA 542 Autosampler: Installation.