Detector Control
Switching the Wavelength

Wavelength Switching is performed by changing the Wavelength (UV detector) or EmWavelength and ExWavelength (fluorescence detector) signal parameters at defined retention times.

For each wavelength switch, enter the time, the channel, and the new parameter value in the Program. This is to detect each peak in the chromatogram at an optimum wavelength.  

Example: The excitation wavelength for the channel of a fluorescence detector switches from 240 nm to 200 nm at 0.600 min.

 

0.530

Emission.ExWavelength

= 240 [nm]

0.600

Emission.ExWavelength

= 200 [nm]

 

Tips:

For most detectors, you can define wavelength switching in the Program Editor. Open the Device View for the detector and go to the Timetable tab page. Append lines to the table and define a timetable for switching the wavelengths.

If you have recorded a 3D field, the Optimum Integration Path may help you to decide the optimum times for switching the wavelength.