Commands and Tips for Third-Party Devices
Agilent 1100 HPLC System: Troubleshooting

• After installation of a device LAN card, the device in which the card is installed may not initialize properly during setup.

  This may occur when each device in your HPLC system has a different firmware version. Perform a firmware update to make sure that all devices are running the same version of the main firmware.

• The connect commands of the Chromeleon server are not completed successfully.

  See the troubleshooting section of  Installing the BootP Server.

• The re-connect commands of the Chromeleon server are not completed successfully.

  Make sure that the J410x LAN card setting is NETWORK AUTO. To do so, configure the settings of the G1323B control module as follows:

• 1. Press Views->System->Configure on the G1323B control module's display.

  2. Select the device in which the LAN card is installed.

  3. Open Interfaces->MIO.

  4. Press Continue.

  5. Press Service.

  6. Select the line displaying CFG NETWORK NO

  7. Press ENTER and select YES. A line showing the network configuration (NETWORK XXXX) is displayed, whereby XXXX is a placeholder for the currently selected setting. Proceed as follows to change the setting to AUTO:

  8. Select the line NETWORK XXXX and press ENTER.

  9. Select AUTO and press ENTER again.

  10. Press Done. Repeat this step a second time.

  11. Press OK to confirm your settings.

  The module will now reset.

• After a sample run starts, one or more devices in the HPLC system turn off.

  This may occur when you are using a refractive index detector and one or more devices in your HPLC system are running outdated firmware. Perform a firmware update from the ChemStation to make sure that all devices are running firmware version A.05.11 or higher.

• An error occurs if any of the devices in the HP 1100 HPLC system is turned off while it is still connected; for example:

  [Abort] 08:12:04 {UV} Fatal device notification (configuration change)

  Re-start the Chromeleon server.

• If the pump stops delivering and the red LED lights, although no leak or any other obvious hardware problem is apparent, this may be due to the settings selected for checking the solvent liquid level. This also applies if the pump does not return to the Ready state after Motor = On and the yellow LED remains lighted. For more information about these commands, refer to Commands and Tips for Third-Party Devices  Agilent 1100 HPLC System: Checking the Solvent Liquid Level.

• If the Communication error: LICOP command not acknowledged. (RE0503 command unknown (outdated firmware?)) error message appears after the pump is installed, select the Disable Sovent Limit Features check box on the Options tab page.

• The following message may appear for detectors:

  [Warning] 15:10:47 1.257 {UV} Raw data delivered by detector has ended prematurely. Finishing acquisition on all channels.

  Detectors in the 1100 HPLC system sometimes do not record data until the end of the program. Data acquisition may end approximately 0.3 minutes before the AcqOff command; thus, raw data is not available in Chromeleon up to the end of the program. (Note: This corresponds to the ChemStation behavior.) To avoid this, do not perform the End command until at least 0.5 minute after the AcqOff command:

  0.000 Inject

  Wait UV.Ready and ColumnComp.Ready and Sampler.Ready and Pump.Ready

  UV_VIS_1.AcqOn

  (…)

  7.500 UV_VIS_1.AcqOff

  8.000 End

   

• When connecting, the 1100 HPLC system sends different device notifications to the Audit Trail. These notifications are usually for informational purposes; however, they may facilitate troubleshooting in the event of system failure.

• The fluorescence detector may remain in the Not Ready state after the detector and the lamp are turned on. In this case, the yellow LED on the detector is on and Chromeleon displays the following message: Initializing excitation monochromator, initializing emission monochromator. Execute the Fluorescence.Reset command to remedy the situation.

• If your system does not reach the Ready state, check the NotReadyCauses property and take appropriate remedial action.

• Dionex recommends performing blank runs with injection. Specify the position of a vial that contains solvent, and then specify the injection command as follows:

  Inject Blank=Inject

  The sampler is running also, thus improving synchronization of the entire 1100 HPLC system.