Dionex Autosamplers
Dionex ASI-100 Autosampler Series
In addition to the standard autosampler commands (see Dionex Autosamplers), the autosamplers of the Dionex ASI-100 series provide many additional commands for controlling autosampler functions, such as the speed with which a sample is drawn (= DrawSpeed), the repeat count for mixing operations (= MixRepeat), or how deep the needle dips into the vial for the washing operations (= WashHeight). You can specify the standard commands further, for example, via the vial whose contents you wish to prepare (= PrepSubject) or via the volume to be used for the Draw, Dispense, and Mix (= PrepVolume) commands. For a list of the supported commands and properties, refer to the table below. (Please note that the display Filter level determines which commands and properties are displayed.)
Special Commands and Properties
Command/Property |
Description |
AcquireExclusiveAccess |
Assigns exclusive access to the current timebase when the autosampler is shared between two timebases. (Also, see ReleaseExclusiveAccess). |
BlueSegment |
Select the segment type that is used in the blue segment. The following types are available: Analytical - Semiprep - Mini - Eppendorf. |
CoolingPower |
Indicates the cooling power (for diagnosis purposes only). |
DispSpeed |
Specify the speed at which the contents of the syringe are dispensed. |
DownSpeed |
Specify the speed at which the needle moves down. |
DrawSpeed |
Specify the speed at which the syringe is filled. |
ExclusiveAccess |
Reports which timebase has exclusive access to an autosampler that is shared by two timebases (read-only). |
ExternalMSV |
State of the external valve. The property is available only if the External MSV installed check box has been selected on the Segments & MSV tab page in the Server Configuration program. |
GreenSegment |
Select the segment type that is used in the green segment. Available types are: Analytical - Semiprep - Mini - Eppendorf. |
HeatSinkTemperature |
Indicates the heat sink temperature (read-only). |
InjectionCounter |
Number of injections. |
InjectMode |
Inject Mode. If set to Normal, the autosampler draws the specified volume from the specified position and injects. If set to Mix, the autosampler injects whatever volume is left in the syringe after preceding draw/dispense operations. |
InjectWaitTime |
Period between the issuing of the command by Chromeleon and the acknowledgement from the autosampler. |
InternalMSV |
State of the internal motorized switching valve. |
Mix |
From the position that is determined via the PrepSubject parameter, the syringe draws and dispenses the volume that is determined via the PrepVolume parameter. If PrepVial is selected as PrepSubject, the PrepVial parameter must be specified as well. The MixRepeat parameter indicates the number of replicates during mixing. |
MixRepeat |
Repeat count for draw, dispense, and mix operations for mixing. |
Msv2ToInject |
Switch the external valve to Inject. |
Msv2ToLoad |
Switch the external valve to Load. |
MsvToInject |
Switch the injection valve to Inject. |
MsvToLoad |
Switch the injection valve to Load. |
NeedleSealCounter |
Needle seal wear. |
PrepareNextSample |
Starts the injection procedure for the next sample, if available. However, the injection valve is only switched at the next Inject command (Display Filter level: Advanced). |
PrepareThisSample |
Starts the injection procedure for the current sample. However, the injection valve is switched only at the next Inject command (Display Filter level: Advanced). |
PrepHeight |
Needle height for draw, dispense and mix operations, specify how deep the needle will dip into the vial for mixing. |
PrepSpeed |
Syringe speed for draw and dispense operations for mixing. |
PrepSubject |
Specify the subject to be used for draw, dispense, and mix operations. The following subjects are available: PrepVial, SampleVial, WashVial, Air, ReagentAVial, ReagentBVial, ReagentCVial, ReagentDVial. If Air is specified as mix subject, air is drawn. During Dispense, the needle moves into the needle port and is dispensed there. |
PrepVial |
Specify the vial position if PrepVial is selected as PrepSubject (see PrepSubject). |
PrepVolume |
Volume to be used for the Draw, Dispense, and Mix. |
Primes the syringe by flushing it several times. |
|
PumpDevice |
Enter the name of the pump for which you want to synchronize the strokes with the autosampler. (This property is available only if you have selected an entry other than <None> from the Sync Inject with pump drop-down list on the Syringe & Stroke Sync tab page in the Server Configuration program.) |
RadialSpeed |
The speed of the radial needle movement. |
ReagentACapacity |
Specify how often the volume can be drawn from the vial with reagent A (reagent B, C, and D, respectively). |
ReagentAVial |
Position of the reagent A (reagent B, C, and D, respectively) vial - is used only if ReagentAVial has been selected as PrepSubject (see PrepSubject). |
RedSegment |
Select the segment type that is used in the red segment. Available types are: Analytical - Semiprep - Mini - Eppendorf. |
Relay1, 2, 3 or 4 |
The following commands are available: State (indicates or sets the state of the relay), Duration (when set, the relay's state toggles after the specified time), On (turns the relay on), and Off (turns the relay off). |
Relay3Enabled |
Relay 3 mode. If set to Yes, the relay can be controlled by Chromeleon. If set to No, the autosampler controls the relay and the relay indicates, whether the autosampler is operable. |
Relay4Enabled |
Relay 4 mode. If set to Yes, the relay can be controlled by Chromeleon. If set to No, the relay is controlled by the autosampler and indicates injection. |
ReleaseExclusiveAccess |
Releases exclusive access to the current timebase for an autosampler that is shared between two timebases. (Also, see ReleaseExclusiveAccess). |
SampleHeight |
Indicates the height at which the sample is drawn, measured from the vial bottom to the needle tip. |
State |
Indicates that the autosampler has injected. |
SyncWithPump |
Synchronizes the injection with the cycle of a P680A LPG or P680A DGP-6 pump. (This property is available only if you have selected an entry other than <None> from the Sync Inject with pump drop-down list on the Syringe & Stroke Sync tab page in the Server Configuration program.) |
Syringe |
Indicates the volume of the syringe that is installed in the autosampler. |
SyringeCounter |
Indicates the number of syringe movements. |
SyringeDelay |
Specify for how long the needle remains in the vial after loading. |
Temperature |
Define the set temperature of the autosampler's tray and hence of the sample. Click the + character to open the command tree; the following commands are available: Value (= actual temperature, read-only), Nominal (= set temperature), UpperLimit,and Lower Limit. With UpperLimit and LowerLimit, the system aborts the batch and starts emergency handling if the nominal temperature is outside these limits. |
TempCtrl |
The following settings are available: On (= cooling/heating enabled) and Off (= cooling/heating disabled) (comp. Nominal, Value, HeatSinkTemperature, CoolingPower). |
Test |
Moves the needle to the specified vial. If no vial is specified, MixSubject is used (comp. PrepSubject). |
TrayDetection |
Turn tray detection (including manual interference monitoring) on or off. When tray detection is enabled, a home run is executed when the tray is installed. |
UpSpeed |
The speed used to move the needle up. |
The internal MSV is switched to the Load position, if necessary. The volume specified under WashVolume is drawn from the position specified under WashVial and dispensed into the needle port/waste. The WashSpeed and WashHeight parameters are also considered. |
|
WaitInput1, 2, 3, or 4 |
State of the digital input 1, 2, 3, or 4. |
WashHeight |
Wash height = distance between the bottom of the wash vial and the needle tip (= the depth with which the needle will dip into the vial). |
WashSpeed |
The speed with which the syringe draws the wash volume. |
WashVial |
Position of the wash vial. |
WashVialVolume |
Total volume of the wash vial. |
WashVolume |
Specify the volume to be drawn from the wash vial. |
Note:
All autosampler inputs are also available as CM input ports so that other device drivers can use them as well (for example, Remote Inject).
For a basic description of Dionex autosamplers, including the standard commands, refer to Dionex Autosamplers.
For information about how to use the commands in practical operation, refer to Practical Tips for Device Control Autosampler Commands (ASI-100 Series).
For information about how to install the autosamplers of the ASI-100 series, refer to Hardware Installation ASI-100 HPLC Autosampler Series: Installation in the Administrator Help section.