Dionex Autosamplers
Dionex ASI-100 Autosampler Series

In addition to the standard autosampler commands (see  Dionex Autosamplers), the autosamplers of the Dionex ASI-100 series provide many additional commands for controlling autosampler functions, such as the speed with which a sample is drawn (= DrawSpeed), the repeat count for mixing operations (= MixRepeat), or how deep the needle dips into the vial for the washing operations (= WashHeight). You can specify the standard commands further, for example, via the vial whose contents you wish to prepare (= PrepSubject) or via the volume to be used for the Draw, Dispense, and Mix (= PrepVolume) commands. For a list of the supported commands and properties, refer to the table below. (Please note that the display Filter level determines which commands and properties are displayed.)

Special Commands and Properties

 

Command/Property

Description

AcquireExclusiveAccess

Assigns exclusive access to the current timebase when the autosampler is shared between two timebases. (Also, see ReleaseExclusiveAccess).

BlueSegment

Select the segment type that is used in the blue segment. The following types are available: Analytical - Semiprep - Mini - Eppendorf.

CoolingPower
(only ASI-100T/ASI-100PT)

Indicates the cooling power (for diagnosis purposes only).

DispSpeed

Specify the speed at which the contents of the syringe are dispensed.

DownSpeed

Specify the speed at which the needle moves down.

DrawSpeed

Specify the speed at which the syringe is filled.

ExclusiveAccess

Reports which timebase has exclusive access to an autosampler that is shared by two timebases (read-only).

ExternalMSV

State of the external valve. The property is available only if the External MSV installed check box has been selected on the Segments & MSV tab page in the Server Configuration program.

GreenSegment

Select the segment type that is used in the green segment. Available types are: Analytical - Semiprep - Mini - Eppendorf.

HeatSinkTemperature
(only ASI-100T/ASI-100PT)

Indicates the heat sink temperature (read-only).

InjectionCounter

Number of injections.

InjectMode

Inject Mode. If set to Normal, the autosampler draws the specified volume from the specified position and injects. If set to Mix, the autosampler injects whatever volume is left in the syringe after preceding draw/dispense operations.

InjectWaitTime

Period between the issuing of the command by Chromeleon and the acknowledgement from the autosampler.

InternalMSV

State of the internal motorized switching valve.

Mix

From the position that is determined via the PrepSubject parameter, the syringe draws and dispenses the volume that is determined via the PrepVolume parameter. If PrepVial is selected as PrepSubject, the PrepVial parameter must be specified as well. The MixRepeat parameter indicates the number of replicates during mixing.

MixRepeat

Repeat count for draw, dispense, and mix operations for mixing.

Msv2ToInject

Switch the external valve to Inject.

Msv2ToLoad

Switch the external valve to Load.

MsvToInject

Switch the injection valve to Inject.

MsvToLoad

Switch the injection valve to Load.

NeedleSealCounter

Needle seal wear.

PrepareNextSample

Starts the injection procedure for the next sample, if available. However, the injection valve is only switched at the next Inject command (Display Filter level: Advanced).

PrepareThisSample

Starts the injection procedure for the current sample. However, the injection valve is switched only at the next Inject command (Display Filter level: Advanced).

PrepHeight

Needle height for draw, dispense and mix operations, specify how deep the needle will dip into the vial for mixing.

PrepSpeed

Syringe speed for draw and dispense operations for mixing.

PrepSubject

Specify the subject to be used for draw, dispense, and mix operations. The following subjects are available: PrepVial, SampleVial, WashVial, Air, ReagentAVial, ReagentBVial, ReagentCVial, ReagentDVial. If Air is specified as mix subject, air is drawn. During Dispense, the needle moves into the needle port and is dispensed there.

PrepVial

Specify the vial position if PrepVial is selected as PrepSubject (see PrepSubject).

PrepVolume

Volume to be used for the Draw, Dispense, and Mix.

PrimeSyringe

Primes the syringe by flushing it several times.

PumpDevice

Enter the name of the pump for which you want to synchronize the strokes with the autosampler. (This property is available only if you have selected an entry other than <None> from the Sync Inject with pump drop-down list on the Syringe & Stroke Sync tab page in the Server Configuration program.)

RadialSpeed

The speed of the radial needle movement.

ReagentACapacity

Specify how often the volume can be drawn from the vial with reagent A (reagent B, C, and D, respectively).

ReagentAVial

Position of the reagent A (reagent B, C, and D, respectively) vial - is used only if ReagentAVial has been selected as PrepSubject (see PrepSubject).

RedSegment

Select the segment type that is used in the red segment. Available types are: Analytical - Semiprep - Mini - Eppendorf.

Relay1, 2, 3 or 4

The following commands are available: State (indicates or sets the state of the relay), Duration (when set, the relay's state toggles after the specified time), On (turns the relay on), and Off (turns the relay off).

Relay3Enabled

Relay 3 mode. If set to Yes, the relay can be controlled by Chromeleon. If set to No, the autosampler controls the relay and the relay indicates, whether the autosampler is operable.

Relay4Enabled

Relay 4 mode. If set to Yes, the relay can be controlled by Chromeleon. If set to No, the relay is controlled by the autosampler and indicates injection.

ReleaseExclusiveAccess

Releases exclusive access to the current timebase for an autosampler that is shared between two timebases. (Also, see ReleaseExclusiveAccess).

SampleHeight

Indicates the height at which the sample is drawn, measured from the vial bottom to the needle tip.

State

Indicates that the autosampler has injected.

SyncWithPump

Synchronizes the injection with the cycle of a P680A LPG or P680A DGP-6 pump. (This property is available only if you have selected an entry other than <None> from the Sync Inject with pump drop-down list on the Syringe & Stroke Sync tab page in the Server Configuration program.)

Syringe

Indicates the volume of the syringe that is installed in the autosampler.

SyringeCounter

Indicates the number of syringe movements.

SyringeDelay

Specify for how long the needle remains in the vial after loading.

Temperature
(only ASI-100T/ASI-100PT)

Define the set temperature of the autosampler's tray and hence of the sample. Click the + character to open the command tree; the following commands are available: Value (= actual temperature, read-only), Nominal (= set temperature), UpperLimit,and Lower Limit. With UpperLimit and LowerLimit, the system aborts the batch and starts emergency handling if the nominal temperature is outside these limits.

TempCtrl
(only ASI-100T/ASI-100PT)

The following settings are available: On (= cooling/heating enabled) and Off (= cooling/heating disabled) (comp. Nominal, Value, HeatSinkTemperature, CoolingPower).

Test

Moves the needle to the specified vial. If no vial is specified, MixSubject is used (comp. PrepSubject).

TrayDetection

Turn tray detection (including manual interference monitoring) on or off. When tray detection is enabled, a home run is executed when the tray is installed.
If the tray was removed while tray detection was disabled, a self-test must be performed when the tray is installed again. This is to make sure that the sample is injected from the correct position.

UpSpeed

The speed used to move the needle up.

Wash

The internal MSV is switched to the Load position, if necessary. The volume specified under WashVolume is drawn from the position specified under WashVial and dispensed into the needle port/waste. The WashSpeed and WashHeight parameters are also considered.

WaitInput1, 2, 3, or 4

State of the digital input 1, 2, 3, or 4.

WashHeight

Wash height = distance between the bottom of the wash vial and the needle tip (= the depth with which the needle will dip into the vial).

WashSpeed

The speed with which the syringe draws the wash volume.

WashVial

Position of the wash vial.

WashVialVolume

Total volume of the wash vial.

WashVolume

Specify the volume to be drawn from the wash vial.

 

 Note:

All autosampler inputs are also available as CM input ports so that other device drivers can use them as well (for example, Remote Inject).

 

For a basic description of Dionex autosamplers, including the standard commands, refer to  Dionex Autosamplers.

For information about how to use the commands in practical operation, refer to Practical Tips for Device Control  Autosampler Commands (ASI-100 Series).

For information about how to install the autosamplers of the ASI-100 series, refer to Hardware Installation  ASI-100 HPLC Autosampler Series: Installation in the Administrator Help section.