Validation Sample

Samples of known concentration that serve to verify a calibration are referred to as validation samples. In the sample list, they are labeled with the sample Type Validate and have the following symbol: .

Verification is by regular insertion of the validation samples in the normal analysis. The resulting (actual) area values are converted into amounts via the Calibration Function and parameters and are then compared with the expected (nominal) values in the Amount table. The result of the nominal/actual comparison can be displayed as direct amount (Amount Deviation result variable) or as deviation in percent (Rel. Amount Deviation).

Checking can also be performed visually if you display the validation samples (marked by colors) in the calibration curve. They are not considered for calibration, though.

Validation samples can be injected also from vials that are normally used for injection of standard (calibration) samples. Validation samples are not relevant for the calculation of the calibration function!

For more information about validation samples, refer to Integrating Chromatograms and Identifying Peaks:

 Entering the Concentration/Amount of the Validation Sample

 Validating the Calibration Curve