Blank Run Subtraction

A blank run subtraction corrects a signal's (channel's) Raw Data by the raw data of a comparable Blank Run sample. The chromatogram of the blank sample is subtracted point by point from the open chromatogram. However, with a Matrix Blank Sample, the peak areas are determined before they are subtracted from the corresponding peak areas of all samples in the sequence.

 Tips:

Perform blank run subtraction if a reproducible shift in baseline is interfering with peak detection; for example, with gradient elution.

Because both the normal and the blank run sample are saved, you can undo or restore the subtraction at any time.

A special interpolation procedure is used to subtract two 3D fields even if the recording conditions are not identical; for example, different wavelength range or optical resolution. However, this procedure should not be used except in special situations.