Matrix Blank Sample

The sample matrix can considerably influence sample analysis. To consider this, the standard samples should have the same matrix or at least a similar one. If this is not the case, you can use matrix blank samples to eliminate the influence of the sample matrix in the individual samples.

Select Matrix as sample type for the respective blank sample. Matrix blank samples are marked as follows: . For this sample, too, the different peak areas (or peak heights, if these were used for quantification) are determined. Chromeleon automatically subtracts the peak areas (or peak heights) of the matrix blank sample from the corresponding peak areas (peak heights) of all sequence samples. The resulting areas (heights) are then used for all further calculations; for example, for calibration. However, the respective peaks must have been identified for both the unknown sample (or standard sample) and the matrix blank sample.

 Note:

Thus, matrix blank values are treated differently from "normal" Blank Run Samples. For normal blank run samples, the chromatogram is subtracted point by point from the chromatogram of the current sample.

If more than one matrix blank sample was recorded in the sequence, the respective peak areas (peak heights) are averaged and the resulting value is subtracted from the corresponding peak areas (peak heights) of the other samples.

 Tip:

Matrix blank samples are subtracted only if they are evaluated in the same QNT Method. Otherwise, they will not be considered.