Calibration Examples
Calibration Mode: Fixed

To use any standard samples of an existing calibration for determining current unknown samples, perform calibration based on the Fixed Calibration Mode.

 Caution:

Always perform the calibration manually (Calibrate). The Auto-Recalibrate option is not available.

a) User Input:

Sample List

Only unknown samples are entered.

If the sample list is generated using the Sequence Wizard, decide whether to refer to an existing QNT File (which is recommended) or whether to leave out this information. When closing the Wizard, Chromeleon recognizes that the sequence does not contain any standard samples and copies the QNT File with the entire information to the sequence directory. The Fixed mode is selected at the same time.

If no QNT File is defined in the Sequence Wizard, enter the file name in the Method column of the sample list after the QNT File has been created.

QNT Method/Peak Table Tab

If an existing QNT File is copied, a peak table already exists. Change the table according to your requirements.

However, if a new (empty) QNT File is used, a new peak table must be created, also. It is not necessary to enter the Amount because calibration is based on an existing calibration.

QNT Method/General Tab

If an existing QNT File is copied in which the Fixed calibration mode is selected, this mode is automatically selected.

If a new QNT File was generated, select the Fixed mode. On the Calibration tab page, determine the standard samples to be used for the calibration.

QNT Method/Calibration Tab

If a QNT File was copied, this page lists all standard samples to which the QNT-File originally referred.

With each newly created QNT File, no standard samples are listed. Select Insert Standard or Append Standard on the context menu to insert the standard samples to be used for calibration and to evaluate the current samples according to their results.

The standard samples listed here always apply to all samples of a sequence. Press the F4 key or the SHIFT+F4 key combination to display the same maximum number of standard samples.

b) Analysis and Evaluation

Performing the analysis and the evaluation of the individual samples is similar to the previous examples.

For an overview of the different calibration possibilities provided by Chromeleon, refer to How to …:  Calibrating.