Ensuring System Wellness
Wavelength Verification

Use this procedure for the Dionex AD25 and PDA-100 detectors. The Wavelength Verification test verifies the wavelength accuracy of the AD25 or PDA-100 detectors. When this test is run, a holmium oxide filter is placed in the light path and measured wavelengths are compared to theoretical wavelengths for holmium oxide.

1. Verify that there is solvent flowing through the cell, the background absorbance is low, and there are no bubbles in the light path.

2. Open the System Wellness Control Panel for the detector (see  Opening a Wellness Control Panel).

3. Under Diagnostic Tests, wavelength verification, click the Verify script button. The detector runs the test and then reports the results to Chromeleon. The overall results (Passed or Failed) are reported and the theoretical and measured values for three peaks in the holmium oxide spectrum.

If the test fails, run the wavelength calibration (see Wavelength Calibration) and then rerun the verification test.