Ensuring System Wellness
Wavelength Calibration

Use this manual wavelength calibration procedure for the Dionex AD25 and PDA-100 detectors.

When the AD25 power is turned on, the wavelength is automatically calibrated. The PDA-100 wavelength is automatically checked under certain conditions, but is not automatically calibrated.

Manually calibrate the wavelength at the following time:

 

When to Calibrate:

After a failed wavelength calibration check

After a failed wavelength calibration

 

  1. Verify that there is solvent flowing through the cell, the background absorbance is low, and there are no bubbles in the light path.

  2. Open the System Wellness Control Panel for the detector (see  Opening a Wellness Control Panel).

  3. Click the wavelength script button under Calibration. The detector performs the wavelength calibration routine and uploads the results (Pass or Fail) to Chromeleon.

If wavelength calibration fails, refer to the troubleshooting section of the detector operator's manual.

Related Topics

  Performing Device Calibrations