Installing and Controlling Thermo Scientific Devices
Finnigan/TSP AS3500 and AS3000 Autosamplers: Installation

Device Type:

Autosampler with 3x40 or 3x35 positions

Device Driver:

Thermo Scientific: TSP AS3000/AS3500 Autosampler

Hardware Options
Supported:

Sample Tray Cooler (rack cooling)

Column Oven (heating of the column oven)

Sample Preparation Kit (sample preparation option)

 Note:  

The AS3000 autosampler was tested with firmware version 3.31.30.

What is required?

License:

Timebase Class 1

Connection:

A free RS-232 port and the following cable is required:

RS-232 cable (Dionex part no. 8914.0150A); for more information, refer to  TSP Cable.

Control Panel:

AS3500_3000.pan

Hardware Installation

Device Connection:

Use an RS-232 cable to connect the TSP AS3500 and AS3000 Autosamplers.

It is not possible to control the AS3000 and AS3500 Autosamplers via a UCI Universal Chromatography Interface or a USB-to-RS232 adapter. To control the autosamplers, use a free RS-232 port on the PC. If more RS-232 ports are needed, install an RS-232 interface card. (For example, refer to Installing PC Plug-In Cards  Installing the Serial RS-232 Interface Card (Meilhaus).)

 Tip:  

When you install the autosamplers in Chromeleon, verify that the Start and Stop inputs are not connected. This will ensure error-free control of the devices.

Device Settings:

  1. For Menu/Options/Input Polarity:

    Pump Ready Active = HI
    Inj Hold Active = LO

  2. For Menu/Options/Output Polarity:

    Set all to LO.

 Note:

If Inj. Hold Active is set to HI, data acquisition cannot be started.

 

Software Installation

Install the Device Driver in the Chromeleon Server Configuration. (For more information about the program, refer to Software Installation and Communication  The Server Configuration Program.)

  1. Start the Chromeleon server.

  2. Start the Server Configuration program.

  3. Add the autosampler to the timebase. Select Add Device on the Edit or context menu, and then select Thermo Scientific from the Manufacturers list box and TSP AS3000/AS3500 Autosampler from the Devices list box.

  4. The following settings are required:

On the General tab page, select the RS-232 port.

On the Installed Options tab page, enable and/or disable control of the option(s) installed for the AS3500 autosampler.

The Error Levels tab page classifies the severity of any errors that occur. It is generally not necessary to change the default settings.

  1. To control the autosampler, connect the AS3500_3000.pan Control Panel in the Chromeleon client to the corresponding timebase.

Application

If you want Chromeleon to control the oven and the sample tray cooler, set the ColOven and TrayTemp properties to Off on the autosampler front panel Menu/Files/Edit/Injection).

For information about how to use the AS3500 and AS3000 Autosamplers' sample preparation features, refer to  Finnigan/TSP AS3500 and AS3000 Autosamplers: Sample Preparation.

Troubleshooting

For information about troubleshooting, refer to  Finnigan/TSP HPLC SpectraSYSTEMŽ Series: Troubleshooting.

 

Further Information

For more installation details, refer to the autosampler manual.

For information about how to use the AS3500 and AS3000 Autosamplers' sample preparation features, refer to  Finnigan/TSP AS3500 and AS3000 Autosamplers: Sample Preparation.

For an overview of the Thermo Finnigan/ThermoQuest/TSP instruments for which device drivers are available in Chromeleon, refer to  Thermo Scientific.