Commands and Tips for Third-Party Devices
Waters Alliance 2790/2795: Overlapping Sample Preparation

The following program is an example for overlapping sample preparation with the Alliance 2790/2795 HPLC Modules and a Waters 996 or 2996 PDA Detector:

 SampleHeater.TempCtrl =  Off

 ColumnOven.TempCtrl =  Off

 Pressure.LowerLimit =  1.0

 Pressure.UpperLimit =  345.0

 %A.Equate =  "%A"

 %B.Equate =  "%B"

 %C.Equate =  "%C"

 %D.Equate =  "%D"

 3DFIELD.MaxWavelength =  799.6

 3DFIELD.MinWavelength =  190.0

 3DFIELD.BunchWidth =  1.2

 3DFIELD.Step =  1.0

 UV_VIS_1.Wavelength =  272

 UV_VIS_1.Bandwidth =  1.2

 UV_VIS_1.Step =  0.50

 UV_VIS_1.Average =  Off

 ExposureTime =  Auto

 Interpolate656nm =  On

 Flow = 1

 %B = 0.0

 %C = 0.0

 %D = 0.0

 Curve = 5

; Optional for overlapping sample prepration:

;  SampleOverlapped = On

 InjectMode = PartialLoop

; The following commands are used to fill the syringe as follows: 10.0 µl air, 5.0 µl sample, 10 µl reagent, and again 10.0 µl air:

 PrepSubject = Air

 PrepVolume = 10.0

 Draw

 PrepSubject = Sample_Vial

 PrepVolume = 5.0

 Draw

 PrepSubject = 2_F8

; For this example: The reagent is in vial 2_F8 of a 48-vial rack

 PrepVolume = 10.0

 Draw

 PrepSubject = Air

 PrepVolume = 10.0

 Draw

 Mix

; The Mix command is required to complete the sample preparation commands:

0.000 Autozero

 Wait UV.Ready and Sampler.Ready and ColumnOven.Ready  and HPLC_System.Ready

 Inject

 3DFIELD.AcqOn

 UV_VIS_1.AcqOn

5.000 3DFIELD.AcqOff

 UV_VIS_1.AcqOff

 End

 Tips:

The SampleOverlapped = Off command is required to disable overlapping sample preparation.

It is not possible to prepare the first sample after a blank sample or after a run without injection while the previous sample is being analyzed.

The PrepSubject = Air command can be specified only as the first and/or the last PrepSubject command.

For more information, also refer to Practical Tips for Device Control  Overlapping Samples.