Property

Min

Max

Description

BubbleDetection²,

Off

On

Checks the system for gas bubbles in the flow path, can return an error message and perform a bubble reduction procedure.

The following settings are required on the instrument: In the Default method, set the Alarms/Bubble detect field to Alert User, Stop Funct, or StopFlow as described in the instrument's manual on page 1 (Mobile Phase).

Continue

see StopFlow

Curve

convex

concave

Gradient Curve (Default: linear)

Note that the figures used to describe the gradient curve shape in Chromeleon differ from the figures used in the Waters Alliance software.

The table below shows how the Chromeleon (CM) curve figures 1–9 are mapped to the Waters Alliance figures 1–11:

Curve no. CM –> Curve no. Alliance:

n/a –> 1

1 –> 2 (convex)

2 –> 3 (convex)

3 –> 4 (convex)

4 –> 5 (convex)

5 –> 6 (linear, default)

6 –> 7 (concave)

7 –> 8 (concave)

8 –> 9 (concave)

9 –> 10 (concave)

n/a  –> 11

(No equivalences for Alliance figures 1 and 11 in Chromeleon).

 Tip:

If the gradient defined by Curve is steeper than the one defined by the MaxAcceleration parameter, the actually performed gradient can be different from the gradient defined here. In this case, a warning appears. This is always the case for step gradients.

MaxAcceleration²

0.01 min

30.00 min

Sets the time needed by the pump to reach the maximum flow rate of 10 ml/min. The property serves to protect the column from major pressure changes.

It is connected with the MaximumFlowRamp property:

MaxAcceleration =
10 / MaximumFlowRamp

(Default: 0.01 min. The property is visible only on the Advanced level.)

MaximumFlowRamp

0.34

1000.00

Maximum flow rate increase in [ml/min²]. This property serves to protect the column from major pressure changes.

This property is connected with the MaxAcceleration property as follows:

MaximumFlowRamp =
10 / MaxAcceleration

(Default: 1000.00 ml/min². The property is visible only on the Advanced level.)

Pressure.LowerLimit
Pressure.UpperLimit²

0.0 bar

344.70 bar
(= 5000 psi)

Upper and lower pressure limits.

For the pressure limits to take effect, the following setting is required on the instrument: In the Default method, set the Alarms/Min. and Alarms/Max fields to Alert User, Stop Funct, or StopFlow as described in the instrument's manual on page 1 (Mobile Phase). The limits entered last in Chromeleon are valid (not the values set on the instrument).

 Note:

The instrument checks the lower pressure limit only during injection.

Pressure.Value

0.0 bar

380 bar
(= 5500 psi)

Indicates the current pump pressure.

PrimaryPressure

-3.45 bar

380 bar

Pressure after the first piston chamber (The property is read-only and visible only on the Expert level.)

StrokeVolume²

Auto; 25 µl;
50 µl; 100 µl

Volume delivered with each piston stroke (Default: Auto. The property is visible only on the Advanced level. )

StopFlow, Continue

When using these commands for the pump of the Alliance system, please note: When the Stop command is executed while a gradient program is running, the Continue command does not restart the gradient. Instead, the pump is started with constant solvent composition and constant flow rate.

Property

Min

Max

Default

Description

HeliumSparge_A
to
HeliumSparge_D²

0

100

0

If the sparge rate is set to 100% for a specific reservoir, the solvent in this reservoir is helium sparged all the time. If the sparge rate is less than 100%, the corresponding valve turns on and off according to the specified percentage.

Property

Value

Default

Description

Degasser
Mode²

Normal
Continuous
Off

Off

Normal: The degasser turns on and off, depending on the pressure.
Continuous: The degasser is always on.
Off: The degasser is always off.

Property

Min

Max

Description

Degasser
Pressure

-1.0

1.0

Indicates the pressure (in bar) measured by the degasser. (The property is read-only.)