Property

Min

Max

Description

CheckPlateHeight²

Off

On

When set to On, the needle position sensor determines the plate height before the first injection is performed from this plate. The value is checked against the range of values allowed for this plate type. (The default setting is Off. The property is visible only on the Advanced level.)

FullLoopOverfill²

1.0%

99.9%

Only in InjectMode = FullLoop (see below).
Specifies the excess volume drawn to rinse the loop when the loop is filled with the sample. (The default setting is 4 %. The property is visible only on the Advanced level.)

InjectWaitTime

0

1E+7

Indicates the time in seconds between the inject command from Chromeleon and the inject response from the autosampler to Chromeleon. (The property is read-only.)

InjPortWashTime²

0, 1, 2, ... 9999

Specifies the time in seconds for inject port washing. (The default setting is 6. The property is visible only on the Advanced level.)

InjectMode²

PartialLoop; FullLoop

Determines the injection mode. (The default setting is FullLoop.)

FullLoop

The entire loop volume is delivered to the column.

PartialLoop

Only part of the loop volume is delivered to the column.

LoopPressure

-3.45

380

Indicates the pressure (in bar) in the sample loop (The property is read-only and visible only on the Expert level.)

NeedleWashTime²

0, 1, 2, ... 9999

Specifies in seconds how long the exterior of the needle is washed. (The default setting is 15. The property is visible only on the Advanced level.)

PrepDelay³

0.0

Loop
volume

Determines in seconds how long it may take until the loop is completely filled. (The default setting is 0.0. The property is visible only on the Advanced level.)

PrepSubject³

Vial positions Sample_Vial, Air, Sample_Vial ± number (relative addressing)

Determines which vial is used for Draw, Dispense, and Mix operations. If set to Air, the needle first draws air, and then dispenses the air into the needle port. (The default setting is 15. The property is visible only on the Advanced level.)

PrepVolume³

0, 1, 2, ... 9999

Determines the volume [µl] to be used for the Draw, Dispense, and Mix commands. (The default setting is 10.0. The property is visible only on the Advanced level.)

PrimeSyringe

Primes the syringe by flushing it.

 Tip:  

After priming the syringe, always perform a Wait Sampler.Ready command.

PurgeVolume³

0, 1, 2, ... 9999

Determines the volume [µl] to be used for purging. (The default setting is 100. The property is visible only on the Advanced level.)

Ready

NotReady

Ready

State of the autosampler (depends on the state of the sample temperature control if WaitForTemperature = Yes).

SampleHeight²

0.0 mm

20.0 mm

Indicates the distance of the needle tip from the bottom of the sample vial (default: 0.0 mm).

SampleOverlapped²

Off

On

When set to On, the current sample will be prepared while the previous sample is still analysed. (The default setting is Off. The property is visible only on the Advanced level.)

SeekWellBottom²

Off

On

Can be enabled only if SampleHeight = 0.0 (see above).

When set to On, the needle detects the well bottom for a specified plate before the first injection is performed. This value is stored for the subsequent injections from this plate. When a different plate is used, the measurement is performed again. (The default setting is Off. The property is visible only on the Advanced level.)

State

Off

On

Indicates On when the autosampler has injected a sample. (The property is read-only.)

SyringeSpeed²,

Fast (5.0 µl/s)
Normal (2.5 µl/s)
Slow (1.0 µl/s)

Defines the drawing speed of the syringe. The speed depends on the installed syringe type. The values correspond to a standard 250 µl syringe.

WaitForTemperature²

No

Yes

Specifies whether the autosampler waits for the sample temperature control.

Wash

Flushes the autosampler.

 Tip:  

After priming the syringe, always perform a Wait Sampler.Ready command.

WashCycle²

0, 1, 2, ... 9

Sets the number of wash cycles to be performed. (The default setting is 1. The property is visible only on the Advanced level.)

WashFrequency²

Never, EveryInject, EveryWell

Determines when a wash cycle shall be performed. The following options are available: Never, EveryInject, EveryWell.

(The default setting is EveryInject. The property is visible only on the Advanced level.)

WashSequence²

PurgeWash, PurgeWashPump

Determines the wash sequence for the needle. (The default setting is PurgeWash. The property is visible only on the Advanced level.)

Command

Description

Draw³

Draws the PrepVolume from the PrepSubject (see above). (The command is available only on Advanced level.)

 Tips:

Up to 16 Draw commands are allowed per injection.

Always use a Mix command after a sequence of Draw commands.

LoopOffline³

Switches the sample injection valve after the injection. Thus, the loop is no longer part of the flow path. Preparation of the next sample can be started. (The property is visible only on the Advanced level.)

 Tip: 

Make sure to issue the command only after the previous sample has completely left the loop.

Mix³

Starts sample preparation. (The property is visible only on the Advanced level.)

 Tips:

No further Draw commands are allowed after a Mix command.

Always use a Mix command after a sequence of Draw commands.

PrimeSyringe

Fills the syringe with the purge solvent. (The property is visible only on the Advanced level.)

 Tip: 

The command uses the values set for PurgeVolume and WashCycle.