Command

Description

Clean

Cleans the syringe using the previously defined parameters (see below).

Inject

Injects the sample using the previously defined parameters (see below).

Tip:

For these autosamplers, 5µl and 10µl syringes are supported by Chromeleon (also refer to chapter ThermoQuest AS3000 and AI3000 Autosamplers: Installation). According to technical specification, the maximum injection volume must not exceed 50% of the syringe capacity.

Recalibrate

Performs the start procedure again, allowing the autosampler to recalibrate the internal positions, such as injector, wash vials, tray. This command is required when a new tray was installed.

StopInject

Aborts a running injection.

Parameter

Description

AirVolume

Select this parameter to specify the volume of air (0.0 - 3.0 µl) drawn after pulling the syringe needle out of the vial. This reduces evaporation from the syringe needle. The total of AirVolume plus Volume must not exceed the syringe volume.

DrawSpeed

Select this parameter to determine how fast the sample is drawn (slow or fast).

FillStrokes

Select this parameter to determine how often the syringe plunger is moved up and down before the sample is actually drawn up (0...15). This eliminates bubbles and thus, enhances reproducibility.

InjectWaitTime

Set the time between the injection command and the response from the autosampler (0 - 10.000.000 s)

PostInjDelayTime

Use this parameter to determine for how long the syringe remains in the injector after the injection (0 - 63 s).

Note:

You can also use the PostInjDelayTime and PreInjDelayTime properties to specify the needle depth during injection. The following settings are available:

Standard: PostInjDelayTime or PreInjDelayTime > 0.

Minimum: PostInjDelayTime and PreInjDelayTime = 0.

PostWash

Select this parameter to set the number of post-injection cleaning cycles (0 - 15).

PostWashVial

Specify which vial(s) is(are) used for washing the syringe after the injection with the solvent(s): A, B, C, D, A+B, C+D.

PreInjDelayTime

Use this parameter to determine the delay the syringe remains in the injector before the injection (0 - 63 s).

Note:

You can also use the PreInjDelayTime and PostInjDelayTime properties to specify the needle depth during injection. The following settings are available:

Standard: PostInjDelayTime or PreInjDelayTime > 0.

Minimum: PostInjDelayTime and PreInjDelayTime = 0.

PreWash

Select this parameter to set the number of pre-injection cleaning cycles (0 - 15)

PreWashVial

Specify which vial(s) is(are) used for washing the syringe before the injection with the solvents: A, B, C, D, A+B, C+D.

SampleDepth

Specify how deep the needle descends into the vial when drawing the sample for injection (bottom or half).

SampleWash

Select this parameter to determine the number of cleaning cycles with sample before injection (0...15).

State

Indicates the status of the autosampler (read-only):
On = the injection has been performed
Off = the injection has not yet been performed