Commands and Tips for Thermo Scientific Devices
ThermoQuest HS850/HS2000 Headspace Samplers: Creating a Program

Follow the steps below to create a program for the headspace sampler:

• Use the PGM Wizard to create a program for the timebase.

• Add all relevant settings for the headspace sampler in the PGM Editor. The settings must appear before the first time step, i.e., before t = 0.000 min.

• Add a Wait Sampler.Ready AND GC.Ready statement directly before the Inject command.

 Tip:  

The GCCycleTime should exceed the total of the GC's program run time and equilibration time.

Example program for Single mode

The following program is a typical example of a sequence for a headspace sampler (here, the FOCUS GC) in Single mode:

; ================================================================

; Sampler settings

; ================================================================

 ; Inject mode

 InjectMode =  Single

 ; Time program

 IncubationTime = 5 [min]

 GCCycleTime =  12 [min]

 ; Enrichment settings: split volume in two loads

 ; without delay between the loads

 LoadsPerInject = 2

 LoadRepetitionTime = 0 [min]

 ; Shaker 

 Shaker =  On

 ShakerOnTime =  10 [s]

 ShakerOffTime = 30 [s]

 ; Temperatures

 NeedleTemperature =  65 [°C]

 OvenTemperature =  60 [°C]

 StandbyNeedleTemperature = 45 [°C]

 StandbyOvenTemperature = 40 [°C]

 ; Sample flushing

 FlushMode =  Always

 FlushPressure = 2 [kPa]

 ; Inject options

 PreFill=  Off

 FillStrokes =  2

 DrawSpeed =  50.00 [ml/min]

 FillVolume =  1.50 [ml]

 PullupDelay =  2 [s]

 PreInjectDelay = 5 [s]

 PostInjectDelay = 5 [s]

 InjectSpeed =  20.00 [ml/min]

; ============================================================

; GC settings (Thermo FOCUS GC)

; ============================================================

 Inlet.TempCtrl =   On

 Inlet.Temperature.Nominal = 100 [°C]

 GC_1.Temp =   110 [°C]

 GC_1.Step =   0.10 [s]

 GC_1.Average =   Off

 GC_1.Range =    0

 GC_1.Data_Collection_Rate = 10 [Hz]

 GasSaverFlow = 1  0.0

 . . .

; ================================================================

; Injection

; ================================================================

0.000  GC_1.Autozero

 GC.Temperature =  100 [°C]

 Wait Sampler.Ready and GC.Ready

 Inject

 Off

 GC_1.AcqOn

10.000 GC.Temperature =  100 [°C]

 GC_1.AcqOff

 End

Example program for Progressive mode

The program for a headspace sampler in Progressive mode is similar to the above program except for the injection mode and time program settings. The following program lines show the relevant settings for a PGM file in which the first sample of the sequence incubated for 4 minutes, the second sample for 6 minutes, the third for 8 minutes, etc.:

 ; Inject mode

 InjectMode =  Progressive

 ; Time program

 IncubTimeStart = 4 [min]

 IncubTimeOffset = 2 [min]

 GCCycleTime =  12 [min]

For more information aboput the headspace samplers, refer to Hardware Installation  ThermoQuest HS850 and HS2000 Headspace Samplers in the Administrator Help section.