Commands and Tips for Third-Party Devices
Agilent 1100/1200 HPLC System: Example for a Derivatization Program

The Agilent 1100 and 1200 HPLC Systems independently process sample preparation programs. Thus, time control by Chromeleon is not supported. To wait during sample preparation, you can only use the Sampler.Wait Time command.

0.000 UV.Autozero

 Flow = 0.450

 %B = 0.0

 Wait UV.Ready and ColumnComp.Ready and Sampler.Ready and Pump.Ready

 ;Derivatization program:

 Sampler.Valve ValvePosition=Bypass

 Draw Volume=5.0, SyringeSpeed=200, Source=Vial, Vial=11

 Draw Volume=1.0, SyringeSpeed=200, Source=Vial, Vial=12

 Draw Volume=0.0, SyringeSpeed=200, Source=Vial, Vial=14

 ;Draws the sample:

 Draw Volume=1.0, SyringeSpeed=200, Source=SampleVial

 ;Mixes 6 times:

Mix Volume=8.0, SyringeSpeed=Maximum, Repeat=6, Source=Seat

 Draw Volume=1.0, SyringeSpeed=200, Source=Vial, Vial=13

 Draw Volume=0.0, SyringeSpeed=200, Source=Vial, Vial=15

 ;Mixes 3 times:

Mix Volume=9.0, SyringeSpeed=Maximum, Repeat=3, Source=Seat

 Sampler.Wait Time=0.5 ; if required

 Sampler.Valve ValvePosition=MainpassOnStart ;(*)

 ;Wash program:

;you can enter additional steps, e.g., to wash the needle. These steps are performed only after the injection, i.e., while the sample is running.

 Inject

;Executes the commands of the wash and sample preparation programs. Injection to the column is performed only after the line (*) has been reached.

 Flow = 0.450

 %B = 0 

 UV_VIS_1.AcqOn

 UV_VIS_2.AcqOn

In a user-defined wash and/or sample preparation program, verify that you have entered all desired steps. After the Inject has been issued, Chromeleon starts processing the single steps to prepare injection. However, the actual injection is performed only when the Valve ValvePosition=MainpassOnStart command has been reached.

 Tip:  

Keep in mind that you have to enter the Valve ValvePosition=MainpassOnStart command as the last step of the sample preparation program.

Also, refer to  Agilent 1100/1200 HPLC System: Program Tips.