Dionex Autosamplers
Dionex AS-HV Autosampler
In addition to the standard autosampler commands (see Dionex Autosamplers), the Dionex AS-HV Autosampler supports several additional commands for controlling autosampler functions. The following commands are available (please note that the display Filter level determines which commands and properties are displayed).
Special Commands for the AS-HV
Command |
Description |
ArmSpeed |
Set the speed of the sampling arm. If septum-capped vials are loaded, always select Septum (the slowest speed). |
DelayVolume |
The volume of the sample loop. |
GotoPosition |
Moves the needle to the specified sample position. |
Inject |
Injects the sample. |
NeedleDown |
Lowers the needle. |
NeedleHeight |
Sets the distance between the needle and the vial bottom (0 is the lowest needle position; 160 is the highest position). |
NeedleHome |
Moves the needle to the home position. |
PrepareNextSample |
(Sample overlap programs only) Rinses the sample loop or concentrator column and, if a sample loop is installed, loads the next sample onto the loop. Note: Chromeleon ignores the PrepareNextSample command if this is the last sample of a sequence, or if the next program is a blank run. |
Rinse |
Rinses the sample loop and the sampling needle. |
RinseAndPrefill |
Rinses the sample loop and then loads the delay volume onto the loop. |
RinseSource |
Reports the source of wash fluid for the rinse station (read-only). |
SampleLoadingPump |
Reports the sample loading pump selected in the AS-HV configuration properties (read-only). |
SampleLoadingType |
Reports whether sample will be loaded onto a sample loop or a concentrator column (read-only). |
SamplesRackType |
Identifies the type of sample rack installed (read-only). |
SerialNo |
Displays the serial number of the AS-HV (read-only). |
StandardsRackType |
Identifies the type of standards rack installed (read-only). |
WashPumpOff |
Turns off the rinse station’s peristaltic pump (through Relay A). |
WashPumpOn |
Turns on the rinse station’s peristaltic pump (through Relay A). |
Special Commands for the Syringe Pump
The following commands are available when the syringe pump is selected as the AS-HV sample loading pump.
Command |
Description |
FlushAspirateSpeed |
Specifies the speed at which the sampling needle is filled with wash fluid. For details, refer to the Note below. |
RinseVolume |
Specifies the volume of wash fluid used to rinse the sampling needle. |
SampleAspirateSpeed |
Specifies the speed at which the syringe is filled with sample. For details, refer to the Note below. |
SyringeAspirate |
Specifies the speed at which the syringe aspirates sample. |
SyringeDispense |
Specifies the speed at which the syringe dispenses sample. For details, refer to the Note below. |
SyringeReset |
Resets the syringe to its default values. |
SyrPumpPosition |
Reports the position of the syringe pump plunger (read-only). |
SyrPumpStatus |
Reports the status of the syringe pump: whether the pump is busy and whether an error condition has been detected (read-only). |
SyringeSize |
Reports the syringe volume (read-only). |
ValvePosition |
Sets the position of the three-way distribution valve in the syringe pump. |
WashPortDispenseSpeed |
Specifies the speed at which wash fluid is dispensed through the sampling needle. For details, refer to the Note below. |
WasteDispenseSpeed |
Specifies the speed at which liquid is directed to waste. |
Note:
There are two syringe speeds (fast and slow), and each speed has 10 levels. The actual flow rate depends on the command, the speed setting, and the syringe size. For more information, refer to the AS-HV operator’s manual.
Special Commands for the AXP Pump
The following commands are available when the AXP pump is selected as the AS-HV sample loading pump.
Command |
Description |
AxpCurrentSetup |
Reports the AXP pump configuration and the status of several operating parameters (read-only). To display details, click the plus sign beside this command. |
AxpState |
Turns the AXP pump on and off. |
Flow |
Sets the flow rate for the AXP pump. |
Pressure.LowerLimit |
Sets the lower pressure limit for the AXP pump. For details, refer to the Note below. |
Pressure.UpperLimit |
Sets the upper pressure limit for the AXP pump. For details, refer to the Note below. |
Pressure.Value |
Reports the current AXP pump pressure (read-only). For details, refer to the Note below. |
RinseVolume |
Specifies the volume of wash fluid used to rinse the sampling needle. |
Note:
To display this command, click the plus sign beside the Pressure command.
Special Commands for the Internal Peristaltic Pump
The following commands are available when the internal peristaltic pump is selected as the AS-HV sample loading pump.
Command |
Description |
Flow |
Sets the flow rate for the internal peristaltic pump. |
InternalPumpOff |
Turns off the internal peristaltic pump. |
InternalPumpOn |
Turns on the internal peristaltic pump. |
IntPumpDirection |
Specifies the direction of operation for the internal peristaltic pump. |
RinseVolume |
Specifies the volume of wash fluid used to rinse the sampling needle. |
Special Commands for a Relay-Controlled Pump
The following commands are available when an external relay-controlled pump is selected as the AS-HV sample loading pump.
Command |
Description |
Flow |
Sets the flow rate for the relay-controlled pump. |
RinseVolume |
Specifies the volume of wash fluid used to rinse the sampling needle. |
For a basic description of Dionex autosamplers, including the standard commands, refer to Dionex Autosamplers.