Dialog Box:
Sequence Wizard: Unknown Samples

In this step of the Sequence Wizard (see Samples and Sequences  The Sequence Wizard), determine whether the Sequence to be generated contains unknown samples. Select one of the following options:

 

No Unknowns:

You cannot enter samples. (The sequence does not contain unknown samples.)

 

 

Use template:

(=Normal case) The sample name is generated via a template. For available templates, see Sample Name.

 

 

Paste from clipboard:

The sample name can be imported from other lists or documents via the clipboard. Select the corresponding section (for example, an Excel column), copy the content, and insert it into the Sequence Wizard by clicking Paste.

 

 

Import from sequence:

Click Import to select an existing sequence. The sample names of the selected sequence are imported into the new sequence.

 

After you select the Use, Paste or Import option, additional fields are displayed. Use these fields to further define the analysis samples to be generated.

 

Template for Sample Name:

(Only for the Use template option)

Select a template for the name of the standard samples to be created. The template consists of a name that can be freely edited and the following wildcards. Click the > button and select:

#n, #N: Sample number

#p, #P: Sample position

#i, #I: Injection volume

#r, #R: Replicate number

##: Character #

When generating the sequence, a freely selectable combination of wildcards for the sample number, position, replicate number, and injection volume is consecutively added to each name. A wildcard can be used multiple times.

In addition, one of the given options can be selected:

1. Sample#p-#r

2. Sample#p; #r.Replicate

3. Sample#n

Example:

Sample#p-#r generates the following sample names for Number of Vials = 2 and Injection per Vial = 2, as well as for Start Position = 6:

Sample6-1

Sample6-2

Sample7-1

Sample7-2

 

 

Number of Vials:

Number of vials to be used in the sequence.

 

 

Injections per Vial:

Specify how often each sample is to be injected. Each injection requires one entry in the sequence.

 

 

Start Position:

Position of the first unknown sample in the autosampler. When generating the sample list, the position number of the next samples is increased by 1. The sample numbers of the analysis and standard samples are automatically checked to avoid overlapping.

 

 

Injection Volume:

Enter the standard volume to be injected. Possible modifications should be made when the sequence is completely generated.

 

 

Apply:

(Essential) Click Apply to create a new sample list from the information specified. View the result in the Sequence Preview or Rack Preview window on the right.

 

Sequence Preview:

Select this option for a preview of the sequence (including sample name, sample position, and sample volume).

 

 

Rack Preview:

Rack Preview illustrates how you have to arrange your samples in the sample rack. The color assignment is as follows:

  • Light blue: Analysis (or unknown) sample

  • Yellow: Standard sample

  • Red: Error - Analysis sample and standard sample overlap. (Because analysis samples and standard samples are created separately (steps 2 and 3), vial positions may overlap. Any necessary corrections can be made afterward.)

If you use an autosampler for which Rack Preview is not supported, or if no connection is available to the Timebase, an error message appears.