Normalization (Fixed Wavelength)
Normalization with a fixed wavelength is analogous to normalization at a relative maximum.
Instead of the relative maximum, the user can choose the wavelength of the absorption value, which seems suited best for normalization.
Tips:
Note that spectrum maxima on the margin of the spectrum can exceed 100%, due to the definition!
If you use the wavelength of the absolute maximum for normalization, it is possible that this wavelength is on the margin of the spectrum. Often, this will be on an edge. On edges, wavelength fluctuations will have stronger effects. Thus, the results may be less precise. This is especially true for detectors that have an optical bandwidth higher than 5nm.
Also, refer to: