Creating a Tandem Program (including Bypass Mode) for an UltiMate 3000 HPLC SystemCreating and Modifying Programs
Creating a Tandem Program (including Bypass Mode) for an UltiMate 3000 HPLC System
A program for operating an UltiMate 3000 x2 Dual-Gradient HPLC System (consisting of a DGP-3600, WPS-3000SL, TCC-3200 and VWD-3400(RS)) in Tandem Operation mode with enabled Bypass Mode could look as follows:
TempCtrl = On
ColumnOven.Temperature.Nominal = 18.0 [°C]
ColumnOven.Temperature.LowerLimit = 5.0 [°C]
ColumnOven.Temperature.UpperLimit = 85.0 [°C]
EquilibrationTime = 0.5 [min]
ReadyTempDelta = 1.0 [°C]
PumpLeft.Pressure.LowerLimit = 1 [bar]
PumpLeft.Pressure.UpperLimit = 400 [bar]
PumpRight.Pressure.LowerLimit = 1 [bar]
PumpRight.Pressure.UpperLimit = 400 [bar]
PumpLeft.%A.Equate = "%A"
PumpRight.%A.Equate = "%A"
PumpLeft.%B.Equate = "%B"
PumpRight.%B.Equate = "%B"
PumpLeft.%C.Equate = "%C"
PumpRight.%C.Equate = "%C"
PumpLeft.MaximumFlowRampDown = 3.000 [ml/min²]
PumpRight.MaximumFlowRampDown = 3.000 [ml/min²]
PumpLeft.MaximumFlowRampUp = 3.000 [ml/min²]
PumpRight.MaximumFlowRampUp = 3.000 [ml/min²]
AnalyticalPump = "PumpLeft"
ReconditionPump = "PumpRight"
DrawSpeed = 5.000 [µl/s]
DrawDelay = 2000 [ms]
DispSpeed = 2.000 [µl/s]
DispenseDelay = 2000 [ms]
WasteSpeed = 4.000 [µl/s]
SampleHeight = 2.000 [mm]
InjectWash = NoWash
PunctureOffset = 0.0 [mm]
PumpDevice = "PumpLeft"
InjectMode = Normal
FlushOutFactor = 2.0
MaxInjectVolume = 100.000 [µl]
LoopWashFactor = 1.000
Data_Collection_Rate = 2.5 [Hz]
TimeConstant = 0.60 [s]
UV_VIS_1.Wavelength = 254 [nm]
UV_VIS_2.Wavelength = 272 [nm]
0.000 Autozero
PumpRight.Flow = 2.000 [ml/min]
PumpRight.%B = 50.0 [%]
PumpRight.%C = 0.0 [%]
PumpLeft.Flow = 1.000 [ml/min]
PumpLeft.%B = 0.0 [%]
PumpLeft.%C = 0.0 [%]
Wait UV.Ready and ColumnOven.Ready and Sampler.Ready
Inject
UV_VIS_1.AcqOn
UV_VIS_2.AcqOn
PumpRight.Flow = 2.000 [ml/min]
PumpRight.%B = 50.0 [%]
PumpRight.%C = 0.0 [%]
PumpLeft.Flow = 1.000 [ml/min]
PumpLeft.%B = 0.0 [%]
PumpLeft.%C = 0.0 [%]
0.875 InjectValveToLoad
; Sets that the chromatographic flow bypasses the sample loop
; (= bypass mode).
1.000 PumpLeft.Flow = 1.000 [ml/min]
PumpLeft.%B = 0.0 [%]
PumpLeft.%C = 0.0 [%]
2.000 PumpLeft.Flow = 2.000 [ml/min]
PumpLeft.%B = 50.0 [%]
PumpLeft.%C = 0.0 [%]
2.500 UV_VIS_1.AcqOff
UV_VIS_2.AcqOff
PumpLeft.Flow = 2.000 [ml/min]
PumpLeft.%B = 50.0 [%]
PumpLeft.%C = 0.0 [%]
PumpLeft.%B = 0.0 [%]
2.600 PumpRight.Flow = 2.000 [ml/min]
PumpRight.%B = 50.0 [%]
PumpRight.%C = 0.0 [%]
3.100 PumpRight.Flow = 2.000 [ml/min]
PumpRight.%B = 0.0 [%]
PumpRight.%C = 0.0 [%]
PumpLeft.Flow = 2.000 [ml/min]
PumpLeft.%B = 0.0 [%]
PumpLeft.%C = 0.0 [%]
NextColumn
; Switches the column switching valve to the next column.
End