Commands and Tips for Third-Party Devices
Waters 515 Pump: Program Tips
The Program Wizard provides only standard pump templates for creating PGM files.
Hold and Stop Flow commands cannot be executed manually or at program times > 0
Step gradients are not supported
Overlapping flow and eluent composition ramps are not supported
Flow ramps (Flow and %B, %C commands) cannot be executed at negative program times (t < 0)
Flow or gradient ramps must start at program times = 0
Time marks for all ramp commands must be a multiple of 0.1 minutes
The pump does not deliver non-linear eluent gradient curves
The pump can only perform ramps with a minimum length of 0.1 min
Pressure limits cannot be set at program time > 0
Hints for automatically generated programs
The Waters 515 pump does not provide PGM wizard support for customized programs. Therefore, use the PGM wizard to automatically create the basic structure of a program and then edit PGM files manually using the F8 Commands box, if necessary. For more information also refer to Manually Editing a PGM File in the Commands View.
When created automatically, each time step of the PGM may contain duplicate commands for flow and eluent composition. These redundant parameters cannot be processed by the Waters 515 pump and must therefore be eliminated from the PGM script. See the PGM below for details:
Pump.Pressure.LowerLimit = 0 [bar] Pump.Pressure.UpperLimit = 400 [bar] 0.000 UV_VIS_1.Autozero UV_VIS_2.Autozero Flow = 1.00 [ml/min] %B = 50 [%] %C = 0 [%] %D = 0 [%] Wait UV.Ready and Sampler.Ready Inject UV_VIS_1.AcqOn UV_VIS_2.AcqOn ; Flow = 1.00 [ml/min] PGM Wizard generates duplicate flow ; %B = 50 [%] and eluent composition parameters. ; %C = 0 [%] Please eliminate parameters ; %D = 0 [%] from PGM script. 5.000 UV_VIS_1.AcqOn UV_VIS_2.AcqOn Flow = 1.00 [ml/min] %B = 100 [%] End