Commands and Tips for Third-Party Devices
Shimadzu LC-10A, LC-20A and LC-30A HPLC Systems: Inject Synchronization

 

Mode

Injection

Standard Sample

Blank Run Sample

 

Shimadzu sampler installed in Chromeleon

Shimadzu Sampler

(i.e., the program includes the Sampler.Inject command)

WaitForRemoteStart is not allowed.

Chromeleon starts a 1-sample batch (i.e., a batch that contains one sample) on the system controller (SCL). The autosampler draws and injects the sample.

Via the driver's internal inject port, Chromeleon generates an inject response upon the SCL start.

WaitForRemoteStart is not allowed.

Chromeleon starts a 0-sample batch (i.e., a batch that does not contain a sample) on the system controller (SCL).
 

Via the driver's internal inject port, Chromeleon generates an inject response upon the SCL start.

 

No Shimadzu sampler installed in Chromeleon

Manual injection via the SCL's contact input (i.e., an inject port is assigned)

WaitForRemoteStart must be set.

Chromeleon starts a 1-sample batch on the SCL from the position "-1". The SCL waits until the contact is closed.

Chromeleon changes the inject port state upon SCL start.

The manual injection device detects this change and generates an inject response.

WaitForRemoteStart must be set.

Chromeleon starts a 0-sample batch on the SCL. The SCL does not wait until the contact is closed.
 

Chromeleon changes the inject port state upon SCL start.

The manual injection device does not wait for a change of the inject port state. An inject response is generated immediately, instead.

Note:

To perform a manual injection for a single sample in a sequence using the SCL contact input you can use one of the following options:

a) Create sequences with only one sample and start them one by one (not all in a batch).

b) If the sequence contains multiple samples, start the sequence run and then click Stop after the current sample to stop the sequence run after one injection.

c) Set all samples in the sequence to Finished, and change the status to Single one after the other. Then restart the sequence.

Inject response from another sampler (i.e., no inject port is assigned)

WaitForRemoteStart is not allowed.

When the Sample2.Inject command is performed, the other sampler injects as usual.

Upon receipt of the inject response, Chromeleon starts a 0-sample batch (i.e., a batch that does not contain a sample) on the SCL.

WaitForRemoteStart is not allowed.

When the Sample2.Inject command is performed, the other sampler injects as usual.

Upon receipt of the inject response, Chromeleon starts a 0-sample batch (i.e., a batch that does not contain a sample) on the SCL.

 

 

 Tip: 

The two injection modes described in No Shimadzu sampler installed in Chromeleon can be used also when a sampler is installed.