Commands and Tips for Third-Party Devices
Shimadzu LC-2010 HPLC System: Setting Gradient Profiles

For the pumps of the Shimadzu LC-2010 HPLC system, Chromeleon allows you to specify linear or non-linear %-Gradient profiles (gradient curves).

The following curve profiles can be defined:

Linear (0): For linear curves (default setting). Changes in composition of the delivered solvent over time are constant.

Convex1–Convex10: Curves of this type are convex upward. Convex curves cause rapid changes in solvent composition at the beginning of the gradient and slower changes at the end. Slope changes over time become extreme as curves go from 1 (least convex) to 10 (most convex).

Concave1–Concave10: Curves of this type are concave upward. Concave curves cause slower changes in solvent composition at the beginning of the gradient and rapid changes at the end. Slope changes over time become extreme as curves go from 1 (least concave) to 10 (most concave).

The figure below shows the solvent composition profiles of curves 1 through 10, for a change from 0 - 100% B over 10 minutes.

 Notes:  

• The Curve property only affects the composition of the delivered solvent (%-gradient). Flow always remains constant.

• Setting individual Curve values for %B, %C or %D, respectively, is not supported.

• The Curve settings on the LC-2010 are represented in a different fashion than in the PGM script: On the device, Curve settings are displayed at the beginning of a time interval (at time = 0). In the PGM script, in contrast, Curve settings are displayed at a later time together with the defined target values.

For information on how to create user-defined flow and gradient profiles using the PGM Wizard, refer to chapter   Flow/Gradient Options/Multi-Step Gradient.