No Overlay:

Select this option if you do not want to display any additional chromatogram. (This option is selected by default.)

Previous Sample

Select this option to overlay the chromatogram with the chromatogram of the most recently chromatographed sample of this sequence.

Previous Standard:

Select this option to overlay the chromatogram with the chromatogram of the most recently chromatographed standard sample of this sequence

Sample No:

Select this option to overlay the chromatogram with the chromatogram of the selected sample of this sequence. Enter the number of the desired sample.

Fixed Chromatogram:

Select this option to overlay the chromatogram with a chromatogram of any other sequence. Type the path in the input field. Or else, click Browse, and then navigate to the chromatogram.

Time Offset:

Specify the percentage for the time offset in x-direction (to the right).

Signal Offset:

Specify the percentage for the signal offset in y-direction (upwards).