Transmission
When light passes through the UV detector flow cell without restraint, transmission is 100%. Transmission decreases with increasing absorption. For the UVD 340S detector, the maximum transmission value is determined via zero order at a reference wavelength of 630nm.
Tip:
The substance mixture passing through the flow cell must not absorb in the reference wavelength range. If this is the case, either change the reference wavelength or select transmission without reference.