Step Gradient

Immediate changes of the solvent composition (for %-Gradients) or the flow (for Flow Gradients) are referred to as step gradients. Contrary to this continual changes of the solvent composition or flow are referred to as Ramp (or more precisely as ramp gradient). In case of step gradients, the elution conditions change rapidly. The realization of the gradient on the column depends among others on the size of the dead volume between the pump and the column end.

To realize a step gradient, enter two percentage values (also, refer to %B, %C, %D)) or two flow values for the same time:

• Enter the current value (to specify that the composition or the flow of the solvent mixture does not change until this time).

• Enter the new value to which to set the solvent (or the flow) immediately: