Creating a Tandem Program (including Bypass Mode) for a Summit HPLC SystemCreating and Modifying Programs
Creating a Tandem Program (including Bypass Mode) for a Summit HPLC System
A program for operating a Summit x2 Dual-Gradient HPLC System (consisting of a P680-DGP, ASI-100, TCC-100, UVD 340U) in Tandem Operation mode with enabled Bypass Mode could look as follows:
TempCtrl = On
ColumnOven.Temperature.Nominal = 18.0 [°C]
ColumnOven.Temperature.LowerLimit = 5.0 [°C]
ColumnOven.Temperature.UpperLimit = 85.0 [°C]
EquilibrationTime = 0.5 [min]
ReadyTempDelta = 1.0 [°C]
HumidityLeakSensor = Standard
GasLeakSensor = Standard
PumpLeft.Pressure.LowerLimit = 1 [bar]
PumpLeft.Pressure.UpperLimit = 400 [bar]
PumpRight.Pressure.LowerLimit = 1 [bar]
PumpRight.Pressure.UpperLimit = 400 [bar]
PumpLeft.%A.Equate = "%A"
PumpRight.%A.Equate = "%A"
PumpLeft.%B.Equate = "%B"
PumpRight.%B.Equate = "%B"
PumpLeft.%C.Equate = "%C"
PumpRight.%C.Equate = "%C"
PumpLeft.MaximumFlowRamp = 3.00 [ml/min²]
PumpRight.MaximumFlowRamp = 3.00 [ml/min²]
AnalyticalPump = "PumpLeft"
ReconditionPump = "PumpRight"
DispSpeed = 10.00 [µl/s]
DrawSpeed = 10.00 [µl/s]
SampleHeight = 0.50 [mm]
SyringeDelay = 5 [s]
UpSpeed = 10.00 [mm/s]
DownSpeed = 10.00 [mm/s]
RadialSpeed = 20.00 [mm/s]
UV_VIS_1.Wavelength = 254 [nm]
UV_VIS_1.Bandwidth = 1 [nm]
UV_VIS_1.RefWavelength = 600 [nm]
UV_VIS_1.RefBandwidth = 1 [nm]
UV_VIS_1.Step = Auto
UV_VIS_1.Average = On
UV_VIS_2.Wavelength = 272 [nm]
UV_VIS_2.Bandwidth = 1 [nm]
UV_VIS_2.RefWavelength = 600 [nm]
UV_VIS_2.RefBandwidth = 1 [nm]
UV_VIS_2.Step = Auto
UV_VIS_2.Average = On
3DFIELD.RefWavelength = 600.0 [nm]
3DFIELD.RefBandwidth = 1.9 [nm]
3DFIELD.Step = 0.5 [s]
3DFIELD.MinWavelength = 200.0 [nm]
3DFIELD.MaxWavelength = 595.2 [nm]
3DFIELD.BunchWidth = 1.9 [nm]
0.000 Autozero
PumpRight.Flow = 2.000 [ml/min]
PumpRight.%B = 50.0 [%]
PumpRight.%C = 0.0 [%]
PumpLeft.Flow = 1.000 [ml/min]
PumpLeft.%B = 0.0 [%]
PumpLeft.%C = 0.0 [%]
Wait ColumnOven.Ready and Sampler.Ready
Inject
UV_VIS_1.AcqOn
UV_VIS_2.AcqOn
3DFIELD.AcqOn
PumpRight.Flow = 2.000 [ml/min]
PumpRight.%B = 50.0 [%]
PumpRight.%C = 0.0 [%]
PumpLeft.Flow = 1.000 [ml/min]
PumpLeft.%B = 0.0 [%]
PumpLeft.%C = 0.0 [%]
0.240 MsvToLoad
; Sets that the chromatographic flow bypasses the sample loop
; (= bypass mode).
1.000 PumpLeft.Flow = 1.000 [ml/min]
PumpLeft.%B = 0.0 [%]
PumpLeft.%C = 0.0 [%]
2.000 PumpLeft.Flow = 2.000 [ml/min]
PumpLeft.%B = 50.0 [%]
PumpLeft.%C = 0.0 [%]
2.500 UV_VIS_1.AcqOff
UV_VIS_2.AcqOff
3DFIELD.AcqOff
PumpLeft.Flow = 2.000 [ml/min]
PumpLeft.%B = 50.0 [%]
PumpLeft.%C = 0.0 [%]
PumpLeft.%B = 0.0 [%]
2.600 PumpRight.Flow = 2.000 [ml/min]
PumpRight.%B = 50.0 [%]
PumpRight.%C = 0.0 [%]
3.100 PumpRight.Flow = 2.000 [ml/min]
PumpRight.%B = 0.0 [%]
PumpRight.%C = 0.0 [%]
PumpLeft.Flow = 2.000 [ml/min]
PumpLeft.%B = 0.0 [%]
PumpLeft.%C = 0.0 [%]
NextColumn
; Switches the column switching valve to the next column.
End