Manual Injections with Templates

If no autosampler is found in the server configuration, Chromeleon assumes that an AS40 Autosampler is connected to the Pump's Relay 1. If, however, sample is being loaded and injected manually instead of via an AS40, and the program is being created by either an application template or a Virtual Column template selected in the Application Wizard, you must modify the PGM File to support this. The PGM File created by the application template is saved in the sequence created by the Sequence Wizard. The PGM File created by the Virtual Column template can be saved to a new sequence or an existing sequence.

Open the PGM File and locate the AS40 load command. The command typically occurs at the beginning of the timed events, at time -2.300. Below is an example AS40 load command; note that "Pump_Relay_1.Closed" closes the AS40 relay, thereby loading the sample into the loop.

-2.300 Pump_Relay_1.Closed Duration=130.00 ; For AS40 injection. Replace this line with the following for manual injection

A manual injection line has been added to the PGM File immediately after the load command:

;-0.100  Message "Load the sample into the injection loop, press OK to continue"

The semicolon at the beginning of the line deactivates (comments out) the manual injection Message. To reactivate it, remove the semicolon at the beginning of the manual injection message and add one to the beginning of the AS40 load command. Do not change the times preceding the commands, even if they differ from the above examples.

;-2.300  Pump_Relay_1.Closed Duration=130.00 ; For AS40 injection. Replace this line with the following for manual injection

-0.100 Message "Load the sample into the injection loop, press OK to continue"

Save and close the PGM File.