Creating an Equilibration ProgramCreating and Modifying Programs
Creating an Equilibration Program
An equilibration program of a Summit HPLC System (consisting of a P680A LPG, ASI-100 with temperature control, TCC-100 with motorized switching valve (MSV), and UVD 340U) could look as follows:
-12.800 %B = 0.0 [%]
%C = 0.0 [%]
%D = 0.0 [%]
Pressure.LowerLimit = 0 [bar]
Pressure.UpperLimit = 400 [bar]
Pump_Pressure.Ripple.UpperLimit = 3.0 [%]
RearSealLeakLimit = 5
MsvPosition = A
ActiveColumn = Column_A
MaximumFlowRamp = 10000.00 [ml/min²]
Flow = 0.000 [ml/min]
Message "Please carefully observe the following instructions. Please note that disregarding the instructions might cause erroneous results or damage parts of your system."
Message "Open the purge valve screw(s)."
Message "CHECK!!! Make sure that the purge valve screw(s) is(are) open!!!"
Flow = 0.000 [ml/min]
-12.700 Flow = 5.000 [ml/min]
Message "Wait at least 5 minutes while the pump purges with a flow of 5.000 ml/min before clicking the 'OK' button; take care that no air bubbles occur in the corresponding channel(s)."
-12.600 Flow = 5.000 [ml/min]
-12.500 Flow = 0.000 [ml/min]
Message "Close the purge valve screw(s)."
WashSpeed = 10.00 [µl/s]
DispSpeed = 10.00 [µl/s]
Message "Verify that you have placed the 4 mL vials in the correct positions: Solvent position: R99, eluent position: B99. Make sure to fill the vials only to half the vial height."
WashVial = R99
PrimeSyringe
Wait Sampler.Ready
WashVial = B99
PrimeSyringe
Wait Sampler.Ready
MsvToInject
-12.000 Flow = 0.000 [ml/min]
-11.000 Flow = 1.000 [ml/min]
Pressure.LowerLimit = 0 [bar]
Pressure.UpperLimit = 400 [bar]
Lamp = On
-9.000 Sampler.TempCtrl = On
Sampler.Temperature.Nominal = 20 [°C]
Sampler.Temperature.LowerLimit = 4 [°C]
Sampler.Temperature.UpperLimit = 45 [°C]
ColumnOven.TempCtrl = On
ColumnOven.Temperature.Nominal = 18.0 [°C]
ColumnOven.Temperature.LowerLimit = 5.0 [°C]
ColumnOven.Temperature.UpperLimit = 85.0 [°C]
ReadyTempDelta = 1.0 [°C]
-7.000 %B = 0.0 [%]
%C = 0.0 [%]
%D = 0.0 [%]
-6.500 %B = 80.0 [%]
%C = 0.0 [%]
%D = 0.0 [%]
-1.500 %B = 80.0 [%]
%C = 0.0 [%]
%D = 0.0 [%]
-1.000 %B = 0.0 [%]
%C = 0.0 [%]
%D = 0.0 [%]
UV.Disconnect
Delay 10.0
UV.Connect
3DFIELD.Step = 0.5 [s]
UV_VIS_1.Wavelength = 225 [nm]
UV_VIS_1.Bandwidth = 1 [nm]
UV_VIS_1.RefWavelength = 600 [nm]
UV_VIS_1.RefBandwidth = 1 [nm]
UV_VIS_1.Step = Auto
UV_VIS_1.Average = On
UV_VIS_1.Noise.UpperLimit = 0.10 [mAU]
UV_VIS_1.Drift.UpperLimit = 3.0 [mAU/hours]
MinLampIntensity = 250000 [counts/s]
0.000 UV.Autozero
Inject
Pump_Pressure.AcqOn
ColumnOven_Temp.AcqOn
UV_VIS_1.AcqOn
Pump_Pressure.StartEquilibration
UV_VIS_1.StartEquilibration
Wait Pump_Pressure.Equilibration AND 15 <= Sampler.Temperature.Value AND Sampler.Temperature.Value <= 25 AND ColumnOven.Ready AND UV_VIS_1.Equilibration AND LampIntensity >= 250000, Continue, Timeout = 45.000
Pump_Pressure.AcqOff
ColumnOven_Temp.AcqOff
UV_VIS_1.AcqOff
Message "The system was equilibrated successfully."
End