Diagnostics (UltiMate 3000 Series)
Holmium Oxide Test (VWD)

Run the holmium oxide test to test the filter function and verify the wavelength accuracy of the VWD-3x00 detector. The test can be run with a dummy flow cell or a flushed flow cell. However, in order to obtain an adequate result for your configuration, it is recommended to use the same flow cell and lamp(s) that was used for data acquisition.

Required materials and preparatory measures

To run this test, you need an UltiMate 3000 pump and a dummy flow cell. As an alternative, use a flushed flow cell (SST or PEEK standard analytical, semipreparative or micro cell) instead of the dummy cell. In this case, use and additional UltiMate 3000 pump, HPLC-grade water as an eluent, a restriction capillary, and a flow rate of 1ml/min (automatically set during the test).

Install the (dummy) flow cell beforehand so that any changes in the configuration become effective before you start the test. Otherwise, you have to restart the test (Control menu -> Diagnostics) after installation of the flow cell.

 Tip: 

Dionex recommends using the Diagnostics Kit for UltiMate 3000 pumps and detectors (Dionex P/N 6035.3000), which contains all materials required for this test.

How to

  1. On the first test page, click Next> to proceed with the settings required for the test.

     Tip: 

    If the Next> button is not active on the first test page, the pump and/or detector is not connected in Chromeleon or is in Standby mode. Connect both modules to Chromeleon before you continue by selecting the Connected check box. If a module is in Standby mode, also clear the Standby check box.

  2. Optional: Enter a descriptive text in the Comments field, e.g., the reason why the test is performed.

  3. Prepare the detector.

    1. Make sure that a (dummy) flow cell is installed in the detector. If not, install a cell and restart the test (Control menu -> Diagnostics) so that the changes become effective. If a cell is installed, confirm by clicking Done.

    2. Select the lamp type to be used for the test  (UV or VIS lamp or both).

    3. If your flow cell is not a dummy cell: Remove the column and replace with the restrictor capillary. Confirm again with Done.

    4. If your flow cell is not a dummy cell: Select the eluent channel for water.

  4. Clicking Next> takes you to the next test step(s). Follow the instructions on the panel(s).

  5. When calibration is finished, remove the restriction capillary (if installed) and confirm by clicking Done.

  6. Clicking Next> takes you to the last wizard page. To view or print the report with details of the analysis, click Finish and Preview Report. Click Finish to quit the test panel without viewing the report. For information about troubleshooting, click Open Troubleshooting Guide.

 

For general information, refer to  Diagnostics (UltiMate 3000 Series).

For more diagnostic tests for UltiMate 3000 Variable Wavelength Detectors, refer to:

 Calibration Test

 Dark Current Test

 Filter Motor Test

 Filter Test (Second Order)

 Grating Motor Test

 Intensity Test