Simulating Chromatograms
Modeling Gradient Separations

Virtual Column’s gradient predictions are based on gradient data acquired under conditions that equate to zero delay time. This means that the start of the concentration ramp reaches the column at exactly the same moment as the sample, thus eliminating any effects of isocratic elution of the sample before the ramp begins.

If you try to reproduce results obtained in Virtual Column, take into consideration that your results may include a delay time, while those obtained with Virtual Column do not. This should have little effect on the results unless high starting concentrations are used in conjunction with low slopes; in that case, monovalent species with short run times may experience a shift in retention time compared to other species.

 Note:

The Chromeleon Virtual Column Linear Gradient license is required to use Virtual Column to simulate gradient separations.