Chromatogram Simulation (Virtual Column)

Virtual Column is a simulation tool that uses known ion chromatographic retention data to predict new retention data and chromatograms. The known retention data was acquired by Dionex using an appropriate experimental design and then embedded into Virtual Column.

 Note:

Results obtained with Virtual Column are intended to represent typical results for a particular column type. Because no two columns or systems are identical, the results you obtain in an actual analysis may differ somewhat from the Virtual Column predictions.

Virtual Column helps you to answer the following questions:

• What is the best column to use for a particular analysis?

• What eluent should I use for optimum separation of analytes?

• What eluent should I use for the fastest separation of analytes?

• How will changing the temperature of an analysis affect the separation?

Licenses

Virtual Column is an optional Chromeleon component. There are two license options: Isocratic and Linear Gradient. The Isocratic license enables modeling of isocratic separations only; the Linear Gradient license enables modeling of isocratic and linear gradient separations. (The Linear Gradient license is sold only in a Virtual Column Complete package that also includes the Isocratic license.)

Evaluation Mode

If you have not purchased a Virtual Column license, you can use Virtual Column in Evaluation Mode. Most functions are available in Evaluation Mode, with the following exceptions:

• In Evaluation Mode, the selection options are limited to the following analytes:

Anions: Bromide, Chloride, Fluoride, Nitrate, Nitrite, Phosphate, and Sulfate

Cations: Ammonium, Calcium, Lithium, Magnesium, Potassium, Sodium

Carbohydrates: Fucose, Galactosamine, Galactose, Glucosamine, Glucose, Mannose

• In Evaluation Mode, the Save, Open, and Reset All commands are not available.

For details about how to use Virtual Column, refer to  Simulating Chromatograms.